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Storyline:  Nashville In the first episode, the series focuses on the rivalry of Rayna Jaymes and Juliette Barnes. Rayna Jaymes is the established "Queen of Country Music". However, her latest album is not selling well and her tour is playing to half empty venues. Her record label suggests that she open for Juliette Barnes, the young and sexy best selling singer of bubble gum country pop. However, Juliette, seeing Rayna as privileged, purposefully alienates her. Rayna, who dislikes Juliette's style of music, rejects a joint tour out of hand. The two women come into conflict as each tries to get guitarist Deacon Claybourne, Rayna's bandmate and former lover, to sign on to their tour. Rayna's life is further complicated when her estranged father, millionaire businessman Lamar Wyatt, convinces her husband, Teddy Conrad, to run for Mayor of Nashville.
For later episodes through season four, the series follows the lives of country musicians, focusing on three female leads: country superstar Rayna Jaymes, rising problematic star Juliette Barnes, and newcomer singer-songwriter Scarlett O'Connor.
From mid-season five, the series follows the lives of country musicians, focusing on two female leads: rising star Maddie Conrad, Rayna James' daughter, and fading star Juliette Barnes, as well as focusing on recurring characters.